A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing
نویسندگان
چکیده
منابع مشابه
Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR-Cas9 has quickly become the technology of choice for genome editing due to its simplicity, efficiency and versatility. Currently, genome editing in plants primarily relies on deliv...
متن کاملGenome editing using Cas9 nickases.
The RNA-guided, sequence-specific endonuclease Cas9 has been widely adopted as genome engineering tool due to its efficiency and ease of use. Derived from the microbial CRISPR (clustered regularly interspaced short palindromic repeat) type II adaptive immune system, Cas9 has now been successfully engineered for genome editing applications in a variety of animal and plant species. To reduce pote...
متن کاملTargeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery
Realizing the full potential of genome editing requires the development of efficient and broadly applicable methods for delivering programmable nucleases and donor templates for homology-directed repair (HDR). The RNA-guided Cas9 endonuclease can be introduced into cells as a purified protein in complex with a single guide RNA (sgRNA). Such ribonucleoproteins (RNPs) can facilitate the high-fide...
متن کاملHigh efficiency, homology-directed genome editing in C. elegans using CRISPR/Cas9 ribonucleoprotein complexes
High efficiency, homology-directed genome editing in C. elegans using CRISPR/Cas9 ribonucleoprotein complexes Alexandre Paix, Andrew Folkmann, Dominique Rasoloson, Geraldine Seydoux HHMI and Dept of Molecular Biology and Genetics, Johns Hopkins University, School of Medicine, Baltimore MD 21205, USA Running title: Genome editing in C. elegans using CRISPR/Cas9 RNP complexes
متن کاملHigh Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes
Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease Cas9 has become a popular method for genome editing in several organisms. Most HDR protocols rely on plasmid-based expression of Cas9 and the gene-specific guide RNAs. Here we report that direct injection of in vitro-assembled Cas9-CRISPR RNA (crRNA) trans-activating crRNA (tracrRNA) ribonucleoprotein complexes into...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Nanotechnology
سال: 2019
ISSN: 1748-3387,1748-3395
DOI: 10.1038/s41565-019-0539-2